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The 31 P spectra were filtered using an exponential window and 20 Hz line broadening, followed by phasing and baseline correction. It has been shown 15 that the mRNA expression levels of MCT1 in LPS treated macrophages, while initially high 4 h post stimulation , can be reduced to almost zero by 16 h, validating our results. Corresponding author: John D. Briefly, after 1 Hz line broadening and proper phasing of each spectrum using the processor, the TSP peak was designated as the reference compound in the profiler. Upon treatment of the macrophages with indomethacin, the hyperpolarized lactate to pyruvate ratio decreased significantly, further reinforcing that lactate could potentially serve as a biomarker of therapy. B 13 C-NMR spectrum of hyperpolarized signals normalized to pyruvate signal shows elevation of lactate production in LPS-activated macrophages in comparison to control inset is 5x magnification of the lactate spectra. Search articles by 'Justin DeLos Santos'.

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Previous work by members of our group showed the conversion of pyruvate to lactate with elevated lactate levels localized to sites of inflammation in the adjuvant induced arthritis rat model 9.

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They help initiate and recruit other immune cells and are activated and deactivated as they participate in the autoregulatory loop in the inflammatory process. Search articles by 'Mark Van Criekinge'. This dose of the non-steroid anti-inflammatory drug was used for treatment in the hyperpolarized studies. Share this article Share with email Share with twitter Share with linkedin Share with facebook.

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